- Rare drop from Tree Lizards.
- Rare drop from the Egg Burdened.
- Eingyi gives one for free to those infected by a parasitic egg, and will sell them for 1,000 each for the rest of the playthrough. He drops two Vermifuges if killed.
- One is found on a corpse in the Painted World of Ariamis.
The Egg Vermifuge serves as the only means to remove the "Egghead" status effect from the player. This status effect is inflicted by the Egg Burdened enemy, and prevents players from wearing a helmet, decreases the player's flame defense, and reduces the player's souls gained by half.