Burning it at the Far Fire bonfire in Majula results in a bolstering of the player's Estus Flask (similar to the role of Fire Keeper Souls in the original Dark Souls). This can be done until the player's Estus Flask is +5; a +5 Flask being the most potent in terms of health restoration.
- Dropped by the second Old Knight wielding a great hammer upon first entering Heide's Tower of Flame. This enemy does not respawn, but will always drop the Sublime Bone Dust. Using a Bonfire Ascetic will respawn the enemy and make another Sublime Bone Dust available (not present in SotFS).
- On a corpse outside the Executioner Chariot boss arena. The player must jump from the bridge onto a separated platform to obtain the dust (not present in SotFS).
- At the bottom of Sinner's Rise, to the first left as players enter the water (not present in SotFS).
- In an iron chest after The Rotten boss fight. (not present in SotFS)
- In an iron chest after the Old Iron King boss fight, before heading down the stairs to the primal bonfire (not present in SotFS).
- Found in an iron chest in the area between Heide's Tower of Flame and No-man's Wharf, behind a Royal Guard.
- Found in a prison cell in Huntsman's Copse. When heading up the hill to activate the lever to lower the bridge, look to your right and you will see a cell with a hole on its top. Drop inside and there will be a Royal Guard, a corpse holding the Soul Spear sorcery, and an iron chest containing the Sublime Bone Dust.
- In an iron chest in the Shaded Woods, near the Shaded Ruins bonfire. Found past Vengarl's body, next to a Royal Guard. Two Fragrant Branches of Yore are required to take this path. It can be obtained for free by jumping out of a window on the left of the bonfire.
- In an iron chest, guarded by a Royal Guard, in the deep pit in Majula. It is found by going to the left in the room filled with Undead Citizens, where Great Heal was previously found.
- Found in Drangleic Castle. When you first get in the castle, head to the left door at the end of the room where you'll find a petrified Royal Guard. After taking care of the Royal Guard, get in the room and there will be the iron chest containing the fifth and final Bone Dust.