- Two can be found in a side room after climbing down from the rooftops and before the second Lothric Knight, who wields a spear.
- Dropped by Man-grubs.
Upon use, an Undead Hunter Charm is thrown to the ground, releasing a white cloud that affects anyone that is hit by it. Affected targets will have a debuff applied to them that prevents them from using their Estus Flask for 25 seconds. Healing Miracles may still be cast, however.
They may also be thrown at Mimics, forcing them to go into a sleeping state after which they can be looted safely. This effect can be used on Mimics in any state, whether they are waiting for prey or are actively attacking the player, and can be re-applied any number of times, dependent on the number of Undead Hunter Charms being carried.